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🧬 Ribosome Transit: How Ribo-Seq Works

Step 1: Ribosome Binding

Ribosome scans and binds to start codon

Step 2: Elongation

Ribosome moves 3nt per cycle, protecting mRNA

Step 3: Add Inhibitor

Translation inhibitor freezes ribosome in place

Step 4: Nuclease Digestion

Unprotected mRNA is digested away

Step 5: RPF Fragment

~28-30nt protected fragment remains