Interactive visualization of 3-nucleotide periodicity in Ribo-Seq data
In ribosome profiling (Ribo-Seq), actively translating ribosomes protect ~28-30nt RNA fragments. Because ribosomes move 3 nucleotides (one codon) at a time, reads show a characteristic 3-nucleotide periodicity pattern. High-quality Ribo-Seq data shows strong enrichment in one reading frame (typically frame 0), indicating the ribosome's P-site position.